Fig. 6

MiR-124-5p knock down reversed the effect of si-AK003290. a Apoptosis of cardiomyocytes was evaluated using Annexin/VI-PI staining under TSA treatment and miR-124-5p mimic transfection. b, c MDA and LDH amounts in cardiomyocytes were analysed using commercial kit. d The ROS of cardiomyocytes was detected using commercial kit and flow cytometry. e JC-1 staining was used to evaluate the MMP. f Targetscan, DIANA-CDS and miRDB were used to predicted the targets of miR-124. Venn analysis was performed to take intersection of the three set. g David analysis was carried out to analyze and enrich those target genes. We displaced the genes related to survival or apoptosis. h The distribution of AK003290 in different organs was showed. *p < 0.05