Fig. 2
From: Exogenous supply of Hsp47 triggers fibrillar collagen deposition in skin cell cultures in vitro
a Z-stack fluorescence images showing up taken 0.3 μM H47 (green) co-localized with ER (red) in NHDF (Blue: DAPI). The yellow dotted line around the cells indicates the edge of the cells. Scale: 20 μm, b H47 uptake by human skin cells (NHDF, HaCaT and HDMEC) and mouse fibroblast (L929, MEF) cells. Cells were incubated with increasing concentrations of H47 in the medium (0.1 μM–1.0 μM) for 3 h. The % of H47 uptake was obtained by quantifying the percentage of cells showing H47 (EGFP green signal) and ER tracker dye (Red signal). 100% H47 uptake of cells indicate that all cells in culture had co-localized signal. Error bars indicate standard deviation of n = 3 experiments. c Western blot of KDEL receptor in membrane protein fractions of human skin cells and mouse fibroblast cells using KDEL receptor antibody. GAPDH signal indicates equal cell density in cytosolic fraction of the corresponding cells stained with GAPDH antibody. Quantification data using whisker’s plot shows deviation in distribution of KDEL receptor in membrane protein fractions. Error bars indicate standard deviation of n = 3 experiments. Statistical significance was analyzed by Tukey test comparing KDEL receptor signal from different cell types (mean ± SD, ANOVA, *** p < 0.001)