Fig. 3
From: Exogenous supply of Hsp47 triggers fibrillar collagen deposition in skin cell cultures in vitro
a Quantification of collagen deposition using Sirius Red assay in different cell types 24 h after 0.5 μM H47 treatment. TGF-β 1 (TGF-β) and ascorbate of same concentration are used as controls. The plots were normalized to collagen deposition in untreated NHDF. Statistical significance for both b and c was analyzed by Tukey test comparing untreated against TGF-β, ascorbate and H47 treated cells (mean ± SD, ANOVA, *** p < 0.001). Error bars representing standard deviation of 6 independent experiments. b-d Quantification of collagen deposition using Picro Sirius Red assay in b. NHDF, c. HaCat and d. HDMEC cells on days 1,3,5,7,10 and 14. Error bars represent standard deviation from 3 individual experiments