Fig. 4From: Exogenous supply of Hsp47 triggers fibrillar collagen deposition in skin cell cultures in vitroQuantification of H47 stimulated deposition of different collagen subtypes. a. Fluorescence images showing immunostained COL I, III, IV, V and XII in NHDF cells before and after treatment with 0.5 μM H47 (Scale – 250 μm) b. Quantification of deposited COL I, III, IV, V and XII from immuno-stained images in NHDF, L929, MEF, HaCaT and HDMEC cultures. Data correspond to collagen deposition 24 h after H47 treatment and controls. The plots were normalized with MEF cells untreated condition as 1. Error bars represent standard deviation from n-3 experiments. Statistical significance was analyzed by Tukey test comparing non treated against H47 treated cells (mean ± SD, ANOVA, *** p < 0.001)Back to article page