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Fig. 4 | BMC Molecular and Cell Biology

Fig. 4

From: Exogenous supply of Hsp47 triggers fibrillar collagen deposition in skin cell cultures in vitro

Fig. 4

Quantification of H47 stimulated deposition of different collagen subtypes. a. Fluorescence images showing immunostained COL I, III, IV, V and XII in NHDF cells before and after treatment with 0.5 μM H47 (Scale – 250 μm) b. Quantification of deposited COL I, III, IV, V and XII from immuno-stained images in NHDF, L929, MEF, HaCaT and HDMEC cultures. Data correspond to collagen deposition 24 h after H47 treatment and controls. The plots were normalized with MEF cells untreated condition as 1. Error bars represent standard deviation from n-3 experiments. Statistical significance was analyzed by Tukey test comparing non treated against H47 treated cells (mean ± SD, ANOVA, *** p < 0.001)

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