Fig. 1

Hyperspectral autofluorescence reveals metabolic differences between cells expressing different mutant PGRMC1-HA proteins. a Principal component analysis (PCA) followed by linear discriminant analysis (LDA) reveal hyperspectral autofluorescence parameters that significantly discriminate between cell types. The panel provides a three dimensional depiction of the three most significant canonical variables revealed by PCA and LDA as described [44] that differ according to PGRMC1-HA phosphorylation status. The table provides p values from Kolmogorov-Smirnov test pairwise discrimination analysis. b Mean cellular intensity of hyperspectral autofluorescence channel 3 [375 nm(Ex), 450 nm(Em)], which corresponds primarily to flavin emission, is significantly affected by PGRMC1-HA phosphorylation status. Boxplots were generated in SPSS. The table shows Kruskal-Wallis test p values for the pair-wise condition comparisons performed on primary emission data