Fig. 3

NpFR1 reveals differences in cytoplasmic redox status. a NpFR1 flow cytometry results. Scatter plots to the left depict green fluorescence on the x axis, and side scatter on the y axis. In the right panels the y axis represents cell number. The numbers at the upper left of each right panel are the fraction of the population in the respective left hand low fluorescent peak. Vertical arrows show the boundary between cell populations, and the inset numbers in the left of each panel represent the percentage of cells in the less oxidized left hand cell population. The vertical dotted reference line represents the 200 fluorescence intensity units, and the open arrow highlights TM fluorescence. b Boxplots showing mean and sample distribution for % cells in the less oxidized population of replicates of (a). Sample size is n = 3, being one each of independent cell lines 1–3 for WT, DM and TM, and triplicates of the MP cell line. The table shows ANOVA post-hoc Dunnet’s T3 p values for the indicated pairwise comparisons. c Boxplot of median fluorescent intensity (MFI) of the oxidized cell populations from (a). The horizontal dotted reference line and open arrow are identical to (A). The table shows ANOVA post-hoc Bonferroni p values for the indicated pairwise comparisons