Fig. 3

CHD9 does not transactivate the core RUNX2 promoter in HEK293T cells. Transactivation of the core RUNX2 promoter by the Ala2384 and Thr2384 CHD9 variants in HEK293T cells measured by luciferase activity. Exogenous expression of the CHD9 Thr2384 and Ala2384 allele did not cause differential transactivation of the thylacine (T.cyn) and red fox (V.vul) RUNX2 core promoter compared to the empty vehicle control. The Ala2384 allele drove a small but significant increase in red fox RUNX2 promoter transactivation compared with the Thr2384 allele, though this was not significantly different to the empty vehicle. RLU = relative light units. * denotes significant differences (P ≤ 0.05)