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Fig. 4 | BMC Molecular and Cell Biology

Fig. 4

From: Basic biochemical characterization of cytosolic enzymes in thymidine nucleotide synthesis in adult rat tissues: implications for tissue specific mitochondrial DNA depletion and deoxynucleoside-based therapy for TK2-deficiency

Fig. 4

Mitochondrial and cytosolic thymidine kinase activities in rat tissues (a) and Mitochondrial TK2 protein levels (b). Mitochondrial TK2 protein levels were determined by immunoprecipitation followed by western blot analysis using TK2 specific antibodies. The band intensity was quantified and converted to ng TK2 protein per mg mitochondrial protein using recombinant human TK2 as standard. The results are presented as mean ± SD. A representative image from IP-western blot is shown. COX 4 was used as loading control in crude extracts before immunoprecipitation. Numbers 1 to 7 represents liver, heart, brain, kidney, spleen, lung and skeletal muscle

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BMC Molecular and Cell Biology

ISSN: 2661-8850

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