Fig. 3
From: Diazoxide affects mitochondrial bioenergetics by the opening of mKATP channel on submicromolar scale
The effect of diazoxide on matrix pH and V0 of K+ uptake, in rat liver mitochondria. a – The typical changes in matrix pH (ΔpHi) in standard incubation medium with following additions: DZ at 0, 0.5, 100 μM (1–3); 0.5 μM DZ, 1 mM Mg2+ (4); Mg·ATP and DZ added at 0.5 (5), 30 (6) and 100 μM (7); b, c – the changes in ΔpHi and V0 of K+ uptake after sequential blockage of K+ transport by Mg2+ and Mg·ATP in the absence (native mitochondria) and the presence of 0.5 μM DZ; d, e – the shares of ATP-sensitive and ATP-insensitive K+ transport in ΔpHi and V0 of K+ uptake found from the blockage of K+ transport by Mg2+ and ATP in native mitochondria (−Mg·ATP) and the activation of mKATP-channel in the presence of Mg·ATP (+Mg·ATP); DZ was added at 0.5 μM (−Mg·ATP); 0.5, 30 and 100 μM (+Mg·ATP). Additions are shown in the legend. M ± m, n = 6; b, c: * - P < 0.05 vs control (0 DZ); # - P < 0.05 vs. Mg2+; ## - P < 0.01 vs. 0.5 μM DZ; ** - P < 0.01 vs. Mg2+, 0.5 μM DZ; D, E: * - P < 0.05 vs. Mg2+ (native); # - P < 0.05 vs. MgATP (native) ## - P < 0.01 vs. MgATP, 0.5 μM DZ