Fig. 4
From: Diazoxide affects mitochondrial bioenergetics by the opening of mKATP channel on submicromolar scale
Bioenergetic effects of diazoxide: respiratory uncoupling (a) and suppression of ROS production (b-d). a – To assess uncoupling effect respiratory control ratio (RCR) was plotted against the rate of state 4 respiration (JO2) stimulated by diazoxide and valinomycin; b – the rates of oxidant formation found as the increments in DCF fluorescence (1) and mitochondrial uncoupling found as RCR (2) against DZ concentration, μM; c – the rates of ROS formation in tightly coupled mitochondria (RCR 7.5)), and under respiratory uncoupling by diazoxide and valinomycin (RCR 4.0); d – the rates of oxidant formation in mitochondria respiring on succinate (1) and glutamate (2). M ± m, n = 4; B-D: *, # - P < 0.05 vs.0 DZ; **, ## - P < 0.05 vs. 0.05 μM DZ