Fig. 3

RORα response elements (ROREs) of the NCEH1 promoter are directly activated by RORα. a HEK293 cells were transiently transfected with the luciferase reporter plasmid in the presence of pSV-βgal. As a luciferase reporter plasmid, wild-type (wt) or mutant (mt) ROREs with three direct repeats, as found in the NCEH1 promoter region, were used. At 36 h after transfection, HEK293 cells were co-transfected without (pSG5) or with RORα expression vector (pRORα). Luciferase activity in the cell lysates was determined and expressed as fold-change in RORα activation based on luciferase activity. Data are means ± standard errors (n = 4). *, p < 0.05 versus PGVP2 control vector. b HEK293 cells were transfected without (pSG5) or with RORα expression vector (pRORα) plus luciferase-driven wild-type [pNCEH1(− 1689/+ 128) or pNCEH1(− 140/+ 128)-wt] or mutant [pNCEH1(− 140/+ 128)-mt] NCEH1 promoters. Mutations in the promoter included the mutation of RORE2. Data are fold-changes in transactivation relative to basal activity and are reported as means ± standard errors (n = 4). *, p < 0.05