Fig. 2
From: Interaction between NSMCE4A and GPS1 links the SMC5/6 complex to the COP9 signalosome
Characterization of the interaction between NSMCE4A and GPS1 by co-immunoprecipitation. HA tagged NSMCE4A and FLAG tagged GPS1 were expressed or co-expressed in HEK cells and precipitated with anti-HA or anti-FLAG antibodies. (a) NSMCE4A, GPS1 were copurified in FLAG-specific immunoprecipitation. (b) NSMCE1 was also detected in the immunoprecipitation for FLAG tagged GPS1. (c) NSMCE4A, GPS1 were copurified in HA-specific immunoprecipitations (d) CSN1 was also detected in the immunoprecipitation for HA tagged NSMCE4A. FLAG-IP: FLAG-specific immunoprecipitation, HA-IP: HA-specific immunoprecipitation, L: lysate (10.5%), IP: immunoprecipitated fraction