Fig. 4
From: Interaction between NSMCE4A and GPS1 links the SMC5/6 complex to the COP9 signalosome
GPS1 depletion resulted in increased levels of SMC6 localization to laser induced DNA damage. (a) Representative images of cells exposed to laser-induced DNA damage following treatment with control siRNA or GPS1 siRNA. (b) Western blot assessment of GPS1 protein depletion following siRNA treatment. (c) Box and whiskers plot showing the quantification of SMC6 signal intensity at the site of laser-induced DNA damage compared to average signal intensity within the undamaged regions of the nucleus following treatment with control siRNA or GPS1 siRNA. P-value of 0.0006 was determined from a two-tailed Mann Whitney t-test. Whiskers represent the minimum and maximum measurements. Ends of the box are the upper and lower quartiles, and the median is marked by a vertical line inside the box. Size bars = 10 μm