Fig. 6

Tbx2 does not decrease levels of C-terminal phosphorylation of Smad1 and Smad2 a) Embryos were injected with 1 ng tbx2 at the two-cell stage; animal caps were explanted at stage 8.5 and incubated in Activin (2.5 ng/ml), as indicated, until late gastrula stages. Uninjected embryos were used in parallel studies. b Embryos were injected with 1 ng tbx2 at the two-cell stage and animal caps were explanted at stage 8.5 and incubated until late gastrula stages. Uninjected embryos were used in parallel studies. c Quantification of data shown in B, indicating no change in Smad1 protein levels or phosphorylation of Smad1 as a result of ectopic tbx2 expression