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Fig. 1 | BMC Molecular and Cell Biology

Fig. 1

From: Functional analyses of miRNA-146b-5p during myogenic proliferation and differentiation in chicken myoblasts

Fig. 1

Design of the chicken miRNA-146b-5p expression vector and characterization of miR-146b-5p overexpression in primary chicken myoblast (pCM) cells. a The expression vector of piggyBac CMV-GFP-mir146b-5p. The cytomegalovirus and EF1 promoter controlled the expression of GFP-miR146b-5p and the puromycin resistance gene, respectively. b Morphology of regular pCM (rpCM) and pCM cells overexpressing miR-146b-5p (pCM-146b OE cells; scale bar = 100 μm). c mRNA expression profiles of miR-146b-5p were compared between regular pCM and pCM-146b OE cells by qRT-PCR (**p < 0.01)

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BMC Molecular and Cell Biology

ISSN: 2661-8850

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