Functional analyses of miRNA-146b-5p during myogenic proliferation and differentiation in chicken myoblasts

Table 2 List of primer sets for PCR analysis

Gene	Forward	Reverse	Annealing Temp. (°C)	PCR size(bp)
Actin	GATGATATTGCTGCGCTCGT	GTGCTCCTCAGGGGCTACTC	60 °C	618
Pax7	AGGTACCAAGAGACGGGCTC	CTCGGCAGTGAAAGTGGTCC	60 °C	411
MyoD	ACACGTCGGACATGCACTTC	TCTGACTCCCCGCTGTAGTG	55 °C	433
SMAD4	GCCCACCACAACATACTCCT	GCACTTGAGATCGAAGGCGT	60 °C	315
NUMB	GCTGCCCCAACTACTACTGC	ACAGGGCACTAATGCTGTCC	55 °C	310
ADCK3	CTGTGCAGCAAACATGTCCT	GGCATCTTCCATTTCCTTGA	60 °C	366
RRM2B	GGACCTTCCTCACTGGAACA	TCCACTTCAGAGCCCAGTCT	55 °C	308
SGCB	CACGAGTTTCATCTGCCAAA	TCACTTGCACCTTGAACAGC	55 °C	343
CCND3	TTTCTGGATGCTGGAGGTGT	ATGCAGAGCTTCTCCACAGT	60 °C	195
IRF2	AATGCAGAGGGACGACTTCA	ACTGGGTGATGTCTGACGTT	60 °C	301
WNT5A	GATACCGCTTTGCCAAGGAG	GCCTACCTTGCGGAAATCAG	60 °C	224
PDGFRB	AGAGCTAGAGGACAGTGGGA	CATTGGAAGCTCGGATGGTG	60 °C	359
CCNB2	TGAAATGTTGGTGGTAGGGC	GGAACAAGTATGCAAGTAGC	60 °C	209
CDC20	GAGTCCTGAACCTGACCATG	CTGTACAGTGTGTAAGCCCA	60 °C	221
KIF23	CCTTTCTTGTCAGGCCCTCT	TCTGTGAGCACGTTACCCTT	60 °C	348
KPNA2	ACACAGAGCAAGGGGTTACA	TCCAAATTCAGGGCAATGCT	60 °C	332
PLK1	CTGATGCTGTGGTGATGGTG	TCTCAACCTGGGCACGTTAA	60 °C	316
TOP2A	TCAACAAAGGCAGCAAGGTC	GGCTCGATTCATCCTGGAGA	60 °C	348
ID1	TGATCGACTACATCTGGGACC	TCTGAGAAGGTTACGAGCCG	60 °C	251
sno RNA: GGGATGTAAAAAAATACTTGCTATC miR-146b: UGAGAACUGAAUUCCAUAGGCG			60 °C

ISSN: 2661-8850