Table 5 List of bi-level regulated proteins and the experimental conditions under which the proteins were identified
No. | Locus_tag | Locus_tag of ortholog in A. platensis group | Phosphoprotein name | Peptide sequence | P-sites | #Phosphopeptides | #Unique phosphopeptides | #P-sites | Temp. | Regulation ([22oC] vs [35oC]) | Regulation ([40oC] vs [35oC]) | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
pS | pT | pY | Total | 22°C | 35°C | 40°C | ||||||||||
1 | SPLC1_S204380 | NIES39_A07260 | 16S rRNA (uracil(1498)-N(3))-methyltransferase | TGQGKGyVYNSLLLAIGPEGGWtTPEVEEAINRR | Y192y T208t | 5 | 2 | 1 | 1 | 5 | ✓ | up | up | |||
SPLC1_S204380 | NIES39_A07260 | 16S rRNA (uracil(1498)-N(3))-methyltransferase | TGQGKGyVYNSLLLAIGPEGGWTtPEVEEAINRR | Y192y T209t | 1 | ✓ | ||||||||||
SPLC1_S204380 | NIES39_A07260 | 16S rRNA (uracil(1498)-N(3))-methyltransferase | tGQGKGyVYNSLLLAIGPEGGWTTPEVEEAINRR | T186t Y192y | 1 | ✓ | ||||||||||
SPLC1_S204380 | NIES39_A07260 | 16S rRNA (uracil(1498)-N(3))-methyltransferase | TGQGKGYVYNSLLLAIGPEGGWttPEVEEAINRR | T208t T209t | ✓ | ✓ | ✓ | |||||||||
SPLC1_S204380 | NIES39_A07260 | 16S rRNA (uracil(1498)-N(3))-methyltransferase | GYVYNSLLLAIGPEGGWTTPEVEEAINRRFQPVSLGsR | S227s | 1 | ✓ | ||||||||||
2 | SPLC1_S101160 | NIES39_Q01000 | adenylate cyclase | SITNTVLEtGNAILTSDAHVDER | T215t | 2 | 1 | 1 | 2 | ✓ | ✓ | down | down | |||
SPLC1_S101160 | NIES39_Q01000 | adenylate cyclase | SITNTVLETGNAILTsDAHVDER | S222s | 1 | ✓ | ||||||||||
3 | SPLC1_S208580 | NIES39_K04210 | adenylate/guanylate cyclase domain-containing protein | LWQSQNLPVIEMRVGIFtGPIVAGSLGsR | T556t S566s | 1 | 1 | 1 | 1 | 2 | ✓ | down | up | |||
4 | SPLC1_S082160 | NIES39_E03060 | aspartyl/glutamyl-tRNA amidotransferase subunit B | VLEYAVKAARALNCEIAPYsK | S82s | 9 | 8 | 1 | 11 | ✓ | up | up | ||||
SPLC1_S082160 | NIES39_E03060 | aspartyl/glutamyl-tRNA amidotransferase subunit B | IGItRLHMEEDAGKLVHGGsDR | T132t S148s | 1 | 1 | ✓ | |||||||||
SPLC1_S082160 | NIES39_E03060 | aspartyl/glutamyl-tRNA amidotransferase subunit B | LVHGGsDRLAGSTYsMVDFNR | S148s S157s | 1 | ✓ | ||||||||||
SPLC1_S082160 | NIES39_E03060 | aspartyl/glutamyl-tRNA amidotransferase subunit B | LVHGGSDRLAGstYSMVDFNR | S154s T155t | 1 | 1 | ✓ | |||||||||
SPLC1_S082160 | NIES39_E03060 | aspartyl/glutamyl-tRNA amidotransferase subunit B | LHMEEDAGKLVHGGSDRLAGStySMVDFNR | T155t Y156y | 1 | ✓ | ||||||||||
SPLC1_S082160 | NIES39_E03060 | aspartyl/glutamyl-tRNA amidotransferase subunit B | RIVRYLGVsDGNMQEGSLR | S200s | 1 | ✓ | ✓ | ✓ | ||||||||
SPLC1_S082160 | NIES39_E03060 | aspartyl/glutamyl-tRNA amidotransferase subunit B | YLGVsDGNMQEGSLR | S200s | ✓ | |||||||||||
SPLC1_S082160 | NIES39_E03060 | aspartyl/glutamyl-tRNA amidotransferase subunit B | CDVNISVRPVGQKEFGtK | T227t | 1 | ✓ | ||||||||||
SPLC1_S082160 | NIES39_E03060 | aspartyl/glutamyl-tRNA amidotransferase subunit B | VLTDDRtVAQYFEATVAAGADtK | T342t T357t | 2 | ✓ | ||||||||||
5 | SPLC1_S102760 | NIES39_Q02320 | beta-ketoacyl-[acyl-carrier-protein] synthase II | ALStRNDDPLHACRPFDVGR | T214t | 1 | 1 | 1 | 1 | ✓ | up | down | ||||
6 | SPLC1_S208940 | NIES39_K04600 | cell division protein FtsH | LAEEIVFGEEEVTTGAsNDLQQVTRVAR | S486s | 2 | 1 | 1 | 2 | ✓ | up | down | ||||
SPLC1_S208940 | NIES39_K04600 | cell division protein FtsH | LAEEIVFGEEEVTTGASNDLQQVtRVAR | T493t | 1 | ✓ | ||||||||||
7 | SPLC1_S034010 | NIES39_D02960 | crossover junction endodeoxyribonuclease RuvA | LFGNHRTNsDIDNLAGACLDALTLQGAGVLMDDR | S231s | 2 | 1 | 1 | 2 | ✓ | down | down | ||||
SPLC1_S034010 | NIES39_D02960 | crossover junction endodeoxyribonuclease RuvA | LFGNHRTNSDIDNLAGACLDALtLQGAGVLMDDR | T245t | 1 | ✓ | ||||||||||
8 | SPLC1_S205200 | NIES39_A07940 | cytochrome c biogenesis protein | MSETLQTQLYEIAQFANtLVR | T18t | 1 | 1 | 1 | 1 | ✓ | ✓ | up | down | |||
9 | SPLC1_S540320 | NIES39_M01840 | diguanylate cyclase | LRHSAsHDSLTDLWNR | S755s | 3 | 2 | 1 | 4 | ✓ | down | down | ||||
SPLC1_S540320 | NIES39_M01840 | diguanylate cyclase | LtDSVDsHILARLGGDEFTILLENIRDIQEAIDVAER | T821t S826s | 1 | 1 | ✓ | |||||||||
SPLC1_S540320 | NIES39_M01840 | diguanylate cyclase | LTDsVDsHILARLGGDEFTILLENIRDIQEAIDVAER | S823s S826s | 1 | ✓ | ||||||||||
10 | SPLC1_S270810 | NIES39_J05180 | DNA-binding protein | AILDLAFSAISQEIEsGQPVVVRGLGK | S38s | 1 | 1 | 1 | 1 | ✓ | up | up | ||||
11 | SPLC1_S430280 | NIES39_O01770 | D-tyrosyl-tRNA(Tyr) deacylase | sVQDIGGELLVVSQFTLYGDCR | S68s | 3 | 1 | 1 | 3 | ✓ | ✓ | down | up | |||
SPLC1_S430280 | NIES39_O01770 | D-tyrosyl-tRNA(Tyr) deacylase | SVQDIGGELLVVsQFTLYGDCR | S80s | 1 | ✓ | ||||||||||
SPLC1_S430280 | NIES39_O01770 | D-tyrosyl-tRNA(Tyr) deacylase | SVQDIGGELLVVSQFTLyGDCR | Y85y | 1 | ✓ | ✓ | |||||||||
12 | SPLC1_S270380 | NIES39_J05540 | glutamate synthase | tIVYKGMVRSAVLGEFYR | T234t | 2 | 1 | 1 | 2 | ✓ | ✓ | up | down | |||
SPLC1_S270380 | NIES39_J05540 | glutamate synthase | TIVyKGMVRSAVLGEFYR | Y237y | 1 | ✓ | ✓ | ✓ | ||||||||
13 | SPLC1_S082010 | NIES39_E02970 | histidine kinase | LVHAPSSSSIFHItKLMSR | T206t | 2 | 1 | 1 | 2 | ✓ | down | down | ||||
SPLC1_S082010 | NIES39_E02970 | histidine kinase | LVHAPSSSSIFHITKLMsR | S210s | 1 | ✓ | ✓ | |||||||||
14 | SPLC1_S230960 | NIES39_L00910 | histidine kinase | IGLVSAPPEVFLKtPsEMDEATQK | T320t S322s | 4 | 2 | 1 | 1 | 6 | ✓ | ✓ | ✓ | down | up | |
SPLC1_S230960 | NIES39_L00910 | histidine kinase | IGLVSAPPEVFLKTPsEMDEAtQK | S322s T328t | 1 | ✓ | ||||||||||
SPLC1_S230960 | NIES39_L00910 | histidine kinase | IVtYLQSsQQELISVNR | T215t S220s | 1 | 1 | ✓ | |||||||||
SPLC1_S230960 | NIES39_L00910 | histidine kinase | IVTYLQssQQELISVNR | S219s S220s | 1 | ✓ | ✓ | |||||||||
15 | SPLC1_S040030 | NIES39_D03400 | hydrogenase accessory protein HypB | LNsAVIVGDLETDNDAQRLR | S129s | 1 | 1 | 1 | 1 | ✓ | down | up | ||||
16 | SPLC1_S260960 | ARTHRO_390015 | hydroxymethylbilane synthase | EDPADALVVHENHRDKQLDtLPPGAVVGTSSLR | T127t | 1 | 1 | 1 | 1 | ✓ | down | down | ||||
17 | SPLC1_S051730 | NIES39_D06980 | hypothetical protein | FNGDGTLVWAQSIGGSDLDSGNGIAVDDAGNVYATGsFSSR | S285s | 2 | 1 | 1 | 2 | ✓ | up | down | ||||
SPLC1_S051730 | NIES39_D06980 | hypothetical protein | FNGDGTLVWAQSIGGSDLDSGNGIAVDDAGNVYATGSFSsR | S288s | 1 | ✓ | ||||||||||
18 | SPLC1_S130380 | NIES39_R00890 | hypothetical protein | KYSIADMDsTSGDLAVETYEIVAEMITKEVALGGQVASMR | S149s | 3 | 1 | 1 | 3 | ✓ | ✓ | down | down | |||
SPLC1_S130380 | NIES39_R00890 | hypothetical protein | KYSIADMDStSGDLAVETYEIVAEMITKEVALGGQVASMR | T150t | 1 | ✓ | ||||||||||
SPLC1_S130380 | NIES39_R00890 | hypothetical protein | KYSIADMDSTSGDLAVETYEIVAEMItKEVALGGQVASMR | T167t | 1 | ✓ | ✓ | ✓ | ||||||||
19 | SPLC1_S171520 | NIES39_A02180 | hypothetical protein | FIPAFGSAIAASWAFAYTGALGEATCVyFGDLMGGK | Y398y | 2 | 1 | 1 | 2 | ✓ | ✓ | ✓ | down | up | ||
SPLC1_S171520 | NIES39_A02180 | hypothetical protein | FIPAFGsAIAASWAFAYTGALGEATCVYFGDLMGGK | S377s | 1 | ✓ | ||||||||||
20 | SPLC1_S202870 | NIES39_A05660 | hypothetical protein | IWTFEQVQGILyVVVPIRMtVIR | Y56y T64t | 4 | 2 | 1 | 1 | 5 | ✓ | up | down | |||
SPLC1_S202870 | NIES39_A05660 | hypothetical protein | LDMGGLLVyAPVAPTPECIRLVNELVAEYGEVR | Y76y | 1 | ✓ | ✓ | |||||||||
SPLC1_S202870 | NIES39_A05660 | hypothetical protein | LDMGGLLVYAPVAPtPECIRLVNELVAEYGEVR | T82t | 1 | ✓ | ||||||||||
SPLC1_S202870 | NIES39_A05660 | hypothetical protein | LDMGGLLVYAPVAPTPECIRLVNELVAEyGEVR | Y96y | 1 | ✓ | ||||||||||
21 | SPLC1_S120070 | SPLC1_S530570 | hypothetical protein | QLVDLTDAKIVyPITFEERK | Y36y | 2 | 1 | 1 | 2 | ✓ | ✓ | ✓ | up | down | ||
SPLC1_S120070 | SPLC1_S530570 | hypothetical protein | QLVDLtDAKIVYPITFEERK | T30t | 1 | ✓ | ||||||||||
22 | SPLC1_S510820 | NIES39_O06510 | IMP dehydrogenase | LGVTVDIIADLHPINPFNFVMESYAERyPFIYEEQLGR | Y102y | 2 | 1 | 1 | 2 | ✓ | up | down | ||||
SPLC1_S510820 | NIES39_O06510 | IMP dehydrogenase | LGVTVDIIADLHPINPFNFVMESYAERYPFIyEEQLGR | Y106y | 1 | ✓ | ||||||||||
23 | SPLC1_S205080 | NIES39_A07830 | isoaspartyl peptidase | GGVEtVRKSLYQVITEVYGLLEK | T27t | 1 | 1 | 1 | 1 | ✓ | up | up | ||||
24 | SPLC1_S060060 | NIES39_D06730 | lysophospholipase | MLRLEHISKIYPtGtILK | T13t T15t | 3 | 2 | 2 | 4 | ✓ | ✓ | up | down | |||
SPLC1_S060060 | NIES39_D06730 | lysophospholipase | IIAGEVEPTSGEVIKPSSLHIAYLTQEFEVDPARtVREEFWR | T80t | 1 | ✓ | ✓ | |||||||||
SPLC1_S060060 | NIES39_D06730 | lysophospholipase | IIAGEVEPTSGEVIKPSSLHIAYLtQEFEVDPARTVREEFWR | T70t | 1 | ✓ | ||||||||||
25 | SPLC1_S207550 | NIES39_K03280 | membrane protein | IVSLSSIAGAITIAALMIItGQPLPYQIFAIAAGtYVIWRHR | T180t T195t | 2 | 1 | 2 | 3 | ✓ | ✓ | up | down | |||
SPLC1_S207550 | NIES39_K03280 | membrane protein | IVSLSSIAGAITIAALMIItGQPLPyQIFAIAAGTYVIWRHR | T180t Y186y | 1 | ✓ | ✓ | |||||||||
26 | SPLC1_S410530 | MULTISPECIES: HNH endonuclease | QVIAAQLPDGsALVEFVR | S61s | 1 | 1 | 1 | 1 | down | up | ||||||
27 | SPLC1_S542340 | peptidase | VVLVtGIAGFIGSAVARLLISKNQK | T13t | 3 | 2 | 1 | 4 | ✓ | down | down | |||||
SPLC1_S542340 | peptidase | LASENYMKIysHQYNISSVALR | Y163y S164s | 1 | 1 | ✓ | ||||||||||
SPLC1_S542340 | peptidase | LASENYMKIYsHQyNISSVALR | S164s Y167y | 1 | ✓ | ✓ | ||||||||||
28 | SPLC1_S201490 | NIES39_A04370 | phosphate permease | LsGRTFFHRWQLAEALAEESESWQFQPPITR | S445s | 2 | 1 | 1 | 2 | ✓ | down | up | ||||
SPLC1_S201490 | NIES39_A04370 | phosphate permease | LSGRTFFHRWQLAEALAEESESWQFQPPItR | T473t | 1 | ✓ | ||||||||||
29 | SPLC1_S240360 | NIES39_K02580 | phosphoglycerate dehydrogenase | SAVNIPGLYPDALEQLKPYLQLAETLGNLVsQLVGGR | S353s | 1 | 1 | 1 | 1 | ✓ | up | up | ||||
30 | SPLC1_S082540 | NIES39_C04520 | CpcD phycobilisome linker domain protein | VTACVPSQTRIRtQR | T18t | 1 | 1 | 1 | 1 | ✓ | down | down | ||||
31 | SPLC1_S203810 | NIES39_A06620 | type I restriction endonuclease subunit R | LQAVRyKQsFDK | Y606y S609s | 1 | 1 | 1 | 1 | 2 | ✓ | ✓ | ✓ | down | down | |
70 | 44 | 30 | 36 | 15 | 81 | |||||||||||