Fig. 1

SP induces enrichment of CD45⁻c-Kit⁺ CPCs in the RA^{1 day SP + I/R} via high expression of c-Kit. (A) Confocal microscopy images showing c-Kit (green)-expressing CPCs in the RA^{1day I/R} and RA^{1day SP + I/R} groups. c-Kit⁺ CPCs (green), CD45⁺ cells (Red), and DAPI nuclei (blue) are visualized. Scale bars, 10 μm. Images were taken with a 100x oil immersion objective using an inverted Zeiss Axio Observer Z1 microscope. Graph showing the percentage of the number of cells per mm² of c-Kit⁺ CPCs in the RA^{1 day I/R} and RA^{1 day SP + I/R} expressed as a percentage difference compared to the corresponding control. *P < 0.05 versus corresponding control using Student’s t-test. (B) Confocal microscopy images showing c-Kit (green)-expressing CPCs in the LA^{1day I/R} and LA^{1day SP + I/R} groups. (C) Confocal microscopy images showing c-Kit (green)-expressing CPCs in the LV/RV^{1day I/R} and LV/RV^{1day SP + I/R} groups. (D) Confocal microscopy images showing c-Kit (green)-expressing CPCs in the Apex^{1day I/R} and Apex^{1day SP + I/R} groups. Scale bars, 20 μm