Fig. 2

SP/NK₁R signaling reveals cooperativity of c-Kit, GATA4, Oct4, Nanog, and Sox2 protein in the RA^{1 day SP + I/R}. (A and B) Western blot analysis of c-Kit, GATA4, Oct4, Nanog, Sox2, and NK₁R for RA^{1 day sham}, RA^{1 day I/R}, RA^{1 day SP + I/R} and RA^{1 day RP/SP + I/R}. Actin was used as a loading control. (C) IFS analysis showing the expression of NK₁R (red) and c-Kit (green) in RA^{1 day I/R}, RA^{1 day SP + I/R}, and RA^{1 day RP/SP + I/R}. Scale bars, 10 μm