RA1 day SP + I/R EDCs show an increased number of c-Kit+ GATA+ Nkx2.5+ CPCs. (A) Schematic workflow of the protocol for RA1 day I/R and RA1 day SP + I/R EDCs using ex vivo explant outgrowth culture assay as described in the Materials and Methods. (B) Confocal fluorescence images of NK1R-expressing c-Kit+ CPCs in each group. c-Kit (green), GATA4 (red), Nkx2.5 (gray), CD45 (gray), Isl-1 (red), and Hoechst33342 nuclei (blue) are visualized. Scale bars, 20 μm. (C) Graph depicting the percentages of c-Kit+ CPCs in RA1 day I/R and RA1 day SP + I/R EDCs. **P < 0.01 versus corresponding control using Student’s t-test. (D) Diagram indicating the percentages of c-Kit+Nkx2.5+GATA4+(), c-Kit+Nkx2.5−GATA4+(), c-Kit+Nkx2.5+GATA4−(), c-Kit−Nkx2.5−GATA4+(), c-Kit−Nkx2.5+GATA4−(), c-Kit−Nkx2.5−GATA4−() in RA1 day I/R and RA1 day SP + I/R EDCs.