Fig. 2
From: The local hypothalamic–pituitary–adrenal axis in cultured human dermal papilla cells
Response of cultured human DPCs to CRF. The endogenous expression of CRF and its receptors in cultured human DPCs (a) and ORSCs (b) (scale bars = 50 μm, blue: DAPI, green: IgG, CRFR1, or CRFR1/2). c, d Human DPCs were exposed to 10− 6 M CRF for 24 h and assessed fluorescence intensity of CRFR1 and CRFR2 using immunocytochemistry (scale bars = 100 μm, blue: DAPI, green: IgG, CRFR1, red: IgG, CRFR2) and e confirmed using western blots. f, g, h The bands were quantified using ImageJ. p values compared with the normal group by t-tests, *: p < 0.05, **: p < 0.01