Fig. 5
From: The local hypothalamic–pituitary–adrenal axis in cultured human dermal papilla cells
The local HPA axis in cultured human DPCs. a Human DPCs were exposed to 10− 6 M CRF with antagonists for 1 h, and the cAMP level was measured using an ELISA kit. p values compared with the control group by t-tests, #: p < 0.05, ##: p < 0.01. p values compared with the CRF treatment group by t-tests, *: p < 0.05, **: p < 0.01. b POMC mRNA levels with CRF treatment at 6 h (RT-PCR). c POMC protein levels with CRF and antagonist treatment at 6 h (western blot). d The bands were quantified using the ImageJ program. e ACTH concentrations at 24 h. f Cortisol levels at 48 h. Ant; antalarmin, A2B; astressin 2-B, C; CRF. p values compared with the control group by t-tests, #: p < 0.05, ##: p < 0.01. p values compared with the CRF treatment group by t-tests, *: p < 0.05, **: p < 0.01