Alterations in the chondrocyte surfaceome in response to pro-inflammatory cytokines

Jeremiasse, Bernadette; Matta, Csaba; Fellows, Christopher R.; Boocock, David J.; Smith, Julia R.; Liddell, Susan; Lafeber, Floris; van Spil, Willem E.; Mobasheri, Ali

doi:10.1186/s12860-020-00288-9

BMC Molecular and Cell Biology

Table 1 Detailed specification of primary and secondary antibodies employed for western blotting

From: Alterations in the chondrocyte surfaceome in response to pro-inflammatory cytokines

Primary antibody	Supplier	Catalog number	Dilution	Secondary antibody	Supplier	Catalog number	Dilution
Annexin A1	LifeSpan Biosciences	LS-C382041 (polyclonal)	1:500	IRDye 800CW Goat	LI-COR Biosciences	926–32,211	1:10,000
Annexin A1	LifeSpan Biosciences	LS-C382041 (polyclonal)	1:500	Anti-Rabbit IgG	LI-COR Biosciences	926–32,211	1:10,000
LRP1	Abcam	ab92544 (monoclonal)	1:400	IRDye 680RD Goat	LI-COR Biosciences	926–68,070	1:8000
LRP1	Abcam	ab92544 (monoclonal)	1:400	Anti-Mouse IgG	LI-COR Biosciences	926–68,070	1:8000
MMP-1 (C-terminal)	Aviva Systems Biology	ARP42040 (polyclonal)	1:1000	IRDye 800CW Goat	LI-COR Biosciences	926–32,211	1:10,000
MMP-1 (C-terminal)	Aviva Systems Biology	ARP42040 (polyclonal)	1:1000	Anti-Rabbit IgG	LI-COR Biosciences	926–32,211	1:10,000
MMP-13 (middle region)	Aviva Systems Biology	ARP56350 (polyclonal)	1:500	IRDye 800CW Goat	LI-COR Biosciences	926–32,211	1:10,000
MMP-13 (middle region)	Aviva Systems Biology	ARP56350 (polyclonal)	1:500	Anti-Rabbit IgG	LI-COR Biosciences	926–32,211	1:10,000
MMP-3 (middle region)	Aviva Systems Biology	ARP42042 (polyclonal)	1:1000	IRDye 800CW Goat	LI-COR Biosciences	926–32,211	1:10,000
MMP-3 (middle region)	Aviva Systems Biology	ARP42042 (polyclonal)	1:1000	Anti-Rabbit IgG	LI-COR Biosciences	926–32,211	1:10,000
TSP-1	Abcam	ab1823 (monoclonal)	1:500^a;	IRDye 680RD Goat	LI-COR Biosciences	926–68,070^a;	1:5000^a
			1:1000^b	Anti-Mouse IgG^a;
				IRDye 800CW Goat		926–32,210^b	1:10,000^b
				Anti-Mouse IgG^b
VDAC1 (C-terminal)	Aviva Systems Biology	ARP35122 (polyclonal)	1:250	IRDye 680RD Goat	LI-COR Biosciences	926–68,071	1:5000
VDAC1 (C-terminal)	Aviva Systems Biology	ARP35122 (polyclonal)	1:250	Anti-Rabbit IgG	LI-COR Biosciences	926–68,071	1:5000
VDAC2 (N-terminal)	Aviva Systems Biology	ARP35123 (polyclonal)	1:250	IRDye 680RD Goat	LI-COR Biosciences	926–68,071	1:5000
VDAC2 (N-terminal)	Aviva Systems Biology	ARP35123 (polyclonal)	1:250	Anti-Rabbit IgG	LI-COR Biosciences	926–68,071	1:5000
β-actin	LI-COR Biosciences	926–42,210 (monoclonal)	1:500	IRDye 800CW Goat	LI-COR Biosciences	926–32,211	1:10,000
β-actin	LI-COR Biosciences	926–42,210 (monoclonal)	1:500	Anti-Rabbit IgG	LI-COR Biosciences	926–32,211	1:10,000

^aEmployed on total cell lysates. ^bEmployed on conditioned media samples

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ISSN: 2661-8850

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