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Table 1 Detailed specification of primary and secondary antibodies employed for western blotting

From: Alterations in the chondrocyte surfaceome in response to pro-inflammatory cytokines

Primary antibodySupplierCatalog numberDilutionSecondary antibodySupplierCatalog numberDilution
Annexin A1LifeSpan BiosciencesLS-C382041 (polyclonal)1:500IRDye 800CW GoatLI-COR Biosciences926–32,2111:10,000
Anti-Rabbit IgG
LRP1Abcamab92544 (monoclonal)1:400IRDye 680RD GoatLI-COR Biosciences926–68,0701:8000
Anti-Mouse IgG
MMP-1 (C-terminal)Aviva Systems BiologyARP42040 (polyclonal)1:1000IRDye 800CW GoatLI-COR Biosciences926–32,2111:10,000
Anti-Rabbit IgG
MMP-13 (middle region)Aviva Systems BiologyARP56350 (polyclonal)1:500IRDye 800CW GoatLI-COR Biosciences926–32,2111:10,000
Anti-Rabbit IgG
MMP-3 (middle region)Aviva Systems BiologyARP42042 (polyclonal)1:1000IRDye 800CW GoatLI-COR Biosciences926–32,2111:10,000
Anti-Rabbit IgG
TSP-1Abcamab1823 (monoclonal)1:500a;IRDye 680RD GoatLI-COR Biosciences926–68,070a;1:5000a
1:1000bAnti-Mouse IgGa;   
IRDye 800CW Goat 926–32,210b1:10,000b
Anti-Mouse IgGb   
VDAC1 (C-terminal)Aviva Systems BiologyARP35122 (polyclonal)1:250IRDye 680RD GoatLI-COR Biosciences926–68,0711:5000
Anti-Rabbit IgG
VDAC2 (N-terminal)Aviva Systems BiologyARP35123 (polyclonal)1:250IRDye 680RD GoatLI-COR Biosciences926–68,0711:5000
Anti-Rabbit IgG
β-actinLI-COR Biosciences926–42,210 (monoclonal)1:500IRDye 800CW GoatLI-COR Biosciences926–32,2111:10,000
Anti-Rabbit IgG
  1. aEmployed on total cell lysates. bEmployed on conditioned media samples