Fig. 2

Bromide dampens clock gene expression in H9C2 cardiomyocytes. H9C2 cardiomyocytes were incubated with NaBr at indicated doses for 24 h. a RT-qPCR analysis of the mRNA expression levels of Bmal1, Cry1 and Rorα. b Western blot analyses of protein expression levels of BMAL1, CRY1 and RORα. c Densitometric analyses of (b). (d-m) RT-qPCR analysis of the mRNA expression levels of clock genes in serum-shocked H9C2 cardiomyocytes treated with or without 400 μM NaBr. *p < 0.05 and **p < 0.01 vs. NaCl group. n = 3. All the data were represented as the mean ± SD