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Fig. 3 | BMC Molecular and Cell Biology

Fig. 3

From: Bromide impairs the circadian clock and glycolytic homeostasis via disruption of autophagy in rat H9C2 cardiomyocytes

Fig. 3

Bromide inhibits glycolytic gene expression in H9C2 cardiomyocytes. H9C2 cardiomyocytes were treated similar as in Fig. 2a. a RT-qPCR analysis of the mRNA expression levels of Hk2, Pkm2 and Pparα. b Western blot analysis of protein expression levels of HK2, PKM2 and PPARα. c Densitometric determinations of (b). (d-f) RT-qPCR analysis of the mRNA expression levels of Hk2, Pkm2 and Pparα in serum-shocked H9C2 cardiomyocytes treated with or without 400 μM NaBr. *p < 0.05 and **p < 0.01 vs. NaCl group. n = 3. All the data were represented as the mean ± SD

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