Fig. 4

Bromide inhibits autophagy in H9C2 cardiomyocytes. a H9C2 cardiomyocytes were infected with the adenovirus expressing GFP-RFP-LC3 for 24 h, and followed by NaBr stimulation for another 24 h. Magnification: 100×. H9C2 cardiomyocytes were treated similar as in Fig. 2a. b Analysis of the images from the experiment shown in Fig. 4a. to determine the average number of particles per cell. c RT-qPCR analysis of the mRNA expression levels of Ulk1, Gabarapl1 and Atg5. d, e Western blot and densitometric analyses of protein expression levels of LC3, ULK1 and ATG5. f-h RT-qPCR analysis of the mRNA expression levels of Ulk1, Gabarapl1 and Atg5 in serum-shocked H9C2 cardiomyocytes treated with or without 400 μM NaBr. *p < 0.05 and **p < 0.01 vs. NaCl group. n = 3. All the data were represented as the mean ± SD