Fig. 5

Autophagy mediates the inhibitory effect of bromide on the circadian clock and glycolytic gene expression in H9C2 cardiomyocytes. H9C2 cardiomyocytes were treated with 400 μM NaBr in combination with or without 100 nM rapamycin for 24 h. a RT-qPCR analysis of the mRNA expression levels of Bmal1, Cry1 and Rorα. b RT-qPCR analysis of mRNA expression levels of Hk2, Pkm2 and Pparα. c Western blot analysis of protein expression levels of LC3, BMAL1, CRY1, RORα, PKM2, HK2, p-mTOR and mTOR. d Densitometric determinations of BMAL1, CRY1, RORα, e HK2 and PKM2. f Densitometric determination analyses of phosphorylation levels of mTOR. *p < 0.05 and **p < 0.01 vs. NaCl group, ^#p < 0.05 and ^##p < 0.01 vs. NaBr group. n = 3. All the data were represented as the mean ± SD