Fig. 5

Exosomal miR-200c-3p inhibited HCT-116 migration. Cells were treated with control anti-miRNA or miRNA mimics for 24 h, induced a wound, and then treated with LPS together with mock or exosome inhibitor GW4869 for additional 24 h as indicated in the Fig. a Representative images of wound gap at 0 h and 24 h after drug treatment in anti-miRNA-transfected group. Scale bar: 100 μm; b Statistical analysis of wound gap distance (μm) between groups with indicated treatment in (A) (n = 3). Two-way ANOVA was performed. *** p < 0.0001; **** p < 0.0001; c Representative images of wound gap at 0 h and 24 h after drug treatment in miRNA mimic-transfected group. Scale bar: 100 μm; d Statistical analysis of wound gap distance (μm) between groups with indicated treatment in (C) (n = 3). Two-way ANOVA was performed. ** p < 0.01; *** p < 0.0001; **** p < 0.0001