Fig. 6

Exosomal miR-200c-3p inhibited HCT-116 invasion. Cells were treated with control anti-miRNA or miRNA mimics for 24 h, and then transferred to Matrigel-coated Transwell chamber, treated with LPS together with mock or exosome inhibitor GW4869 for additional 24 h as indicated in the Fig. a Representative images of stained invaded cells 24 h after drug treatment in anti-miRNA-transfected group. Scale bar: 50 μm; b Statistical analysis of invaded cell number between groups with indicated treatment in (A) (n = 3). Two-way ANOVA was performed. *** p < 0.001; **** p < 0.0001; c Representative images of stained invaded cells 24 h after drug treatment in miRNA mimic-transfected group. Scale bar: 50 μm; d Statistical analysis of invaded cell number between groups with indicated treatment in (C) (n = 3). Two-way ANOVA was performed. ** p < 0.01; *** p < 0.001; **** p < 0.0001