Fig. 7
Exosomal miR-200c-3p inhibited ZEB-1 expression. a Predicted pairing of human miR-200c-3p to wildtype (WT) and mutated (Mut) Zeb-1 3’UTR and Zeb-2 3’UTR. b Dual luciferase gene reporter assay in HCT-116 cells co-transfected with indicated 3’UTR constructs and miRNA mimics (n = 3). Two-way ANOVA was performed for statistical analysis. N.S., not significant; ** p < 0.01. c Representative Western blot of ZEB-1 and ZEB-2 in anti-miRNA-transfected HCT-116 cell lysates with indicated treatments. GAPDH was used as loading control; d Statistical analysis of band intensities in (C) (n = 3). One-way ANOVA was performed. N.S., not significant; *** p < 0.001; e Representative Western blot of ZEB-1 and ZEB-2 in miRNA mimic-transfected HCT-116 cell lysates with indicated treatments. GAPDH was used as loading control; f Statistical analysis of band intensities in (E) (n = 3). One-way ANOVA was performed. N.S., not significant; * p < 0.05