Fig. 1From: Klotho is regulated by transcription factor Sp1 in renal tubular epithelial cellsOverexpression of Sp1 upregulates Klotho expression. HK-2 cells (a-b) or HEK-293 cells (c-d) were transfected with increasing amount of pcDNA3-Sp1 plasmid or empty control. 48 h after transfection, total proteins were isolated and the protein levels of Sp1 and Klotho were detected by Western blots (a/c). Total cell RNAs were isolated for quantitative RT-PCR to analyze the mRNA level of Klotho (b/d). Data were expressed as the mean ± SD in three independent experiments. ***p < 0.001, **p < 0.01, *p < 0.05. NC, empty vector; C: untreated controlBack to article page