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Fig. 6 | BMC Molecular and Cell Biology

Fig. 6

From: Klotho is regulated by transcription factor Sp1 in renal tubular epithelial cells

Fig. 6

ChIP assay of Sp1 protein binding to the KL promoter. (a) Schematic diagram of KL promoter and control region. Black (target) and white arrow (off target) indicate the position of the ChIP PCR primers. The transcriptional initiation site (arrow) corresponding to the promoter region is considered to be + 1. (b) HK-2 cells were transiently transfected with pcDNA3-Sp1 plasmid or empty control (pcDNA3). 24 h after transfection, cells were cross-linked, sonicated and immunoprecipitated with Sp1 antibody or IgG (negative control). The immunoprecipitated DNA and the input DNA were obtained for PCR testing

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