Fig. 4

Groups of residues involved based on coevolution analysis. a–cBacillus polymyxa β-glucosidase (PDB ID: 1BGA) is represented as a cartoon. The colors yellow, cyan, orange, magenta, and green represent loops a–e, respectively. Catalytic residues (E166 and E352) are presented in purple. The red spheres represent thermostabilizing mutations described for GH1 β-glucosidases in the literature [85, 89] and from a thermophile GH1 structure (PDB: 5IDI). a Blue spheres depict community one residues. b Green spheres depict community two residues. c Graph of coevolving residues and their communities