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Fig. 1 | BMC Molecular and Cell Biology

Fig. 1

From: Differential contribution of p300 and CBP to regulatory element acetylation in mESCs

Fig. 1

p300 Maintains Enhancer Acetylation in mESCs. a Immunoblot of whole cell lysates from mESCs transfected with scramble shRNA or either p300 or CBP shRNA and relative quantification. Data are represented as mean ± S.D. of at least 3 independent experiments. *** p < 0.001 vs Ctl. b Browser track of p300 [19] and CBP [20] in WT mESC and H3K27ac in Control and P300KD or CBPKD mESCs. Promoter and enhancer regions for two important pluripotency transcription factors (Pou5f1 and Sox2) are highlighted in blue. c,d H3K27ac ChIP-seq enrichment in mESCs expressing scramble control (Ctl) shRNA compared with b p300 shRNA (p300 KD) or c CBP shRNA (CBP KD). Blue and red dots represent differential ChIP-seq regions at which read density increased or decreased by 2-fold or more (FDR < 0.05), respectively, in three independent biological replicates. e, f Fold-change ratio (log2) of H3K27ac enrichment at promoters and enhancers in mESCs expressing scramble control (Ctl) shRNA compared with e p300 shRNA (p300 KD) or f CBP shRNA (CBP KD). Data to the right of log2(0) = 1 indicate regions with increased H3K27ac after acetyltransferase knockdown, while data to the left indicate regions with reduced H3K27ac. *** p-value < 2.2e-16 enhancer vs promoter regions

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