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Fig. 4 | BMC Molecular and Cell Biology

Fig. 4

From: Determinants of translation efficiency in the evolutionarily-divergent protist Trichomonas vaginalis

Fig. 4

Details of the synthetic iLOV gene library to be expressed in T. vaginalis.a Diagram of the iLOV genes showing 5′ and 3′ portions connected by the Nru I site. Five distinct folding profiles and three discrete CAI levels were designed for the sequences at the 5′ and 3′ portions, respectively, without altering the protein product. When combined together after gene synthesis and cloning, these 5′ and 3′ segments produced 15 synonymous iLOV genes. b Secondary structures of the five distinct folding profiles were predicted by RNAfold web server (http://rna.tbi.univie.ac.at/cgi-bin/RNAWebSuite/RNAfold.cgi) [42]. The first transcribed nucleotide is pointed on the sequences (− 5′). Color indicates the probability of a particular nucleotide staying in the predicted base-pairing state

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