Fig. 5

Transient and stable expressions of the synonymous iLOV genes determined by flow cytometry. Data were collected for transient and stable expressions of the 15 synonymous iLOV genes, created by the combinations of five folding profiles (Profile_1 to _5) and three CAI levels (H-high, M-medium and L-low) as per Table 2, from three experimental replicates. pMN-empty and iLOV_CO were used as negative and positive controls, respectively. a Transient expression of synonymous iLOV genes in T. vaginalis, 8 h post-electroporation. Green fluorescence intensities of transfectants were detected by FL1, and dead cells excluded by FL3 after being stained by 0.2% propidium iodine. Live cells expressing green fluorescence are located in the quadrant Q3 (bottom right). b Stable expression of synonymous iLOV genes in T. vaginalis after drug selection. One-way ANOVA and Tukey’s range test were conducted to determine the significance of differences between folding profiles and CAI levels (see Additional file: Description of Statistical Analyses)