Fig. 4

Wild-type APP is similarly dependent upon Alix and Syntenin-1 for secretion. a Inducible shRNA knockdown cells were transfected with wild-type APP (APP^WT), equal mass loaded. b EVs were harvested from APP^WT transfected cells for immunoblot analysis, equal volume loaded. c Quantitation of APP^WT secreted into EVs from knockdown cells, normalized to cellular levels. d GFP-tagged wild-type Vps4a and corresponding dominant negative construct (Vps4a E228Q) were transfected into HEK293 cells expressing APP^WT. EVs were collected and analyzed by immunoblot analysis, equal volume loaded. e Quantitation of APP^WT secreted into EVs from wild-type Vps4a and dominant negative Vps4a transfected cells. Blots are representative images from three to five repeated independent experiments. T-test: *, p < 0.05; **, p < 0.01