Fig. 5
From: Alix and Syntenin-1 direct amyloid precursor protein trafficking into extracellular vesicles
Alix-dependent APP trafficking is conserved in neuron-like cells. a SH-SY5Y neuroblastoma cells stably transduced with inducible shRNA targeting Alix were terminally differentiated into neuron-like cells, then transfected with APPswe. Cell lysates and cell-derived EVs were harvested for immunoblot analysis. b Quantitation of APPswe secretion into vesicles. c Representative immunoblots of cellular levels and vesicular secretion of endogenous SH-SY5Y APP. Blots are representative images from two to three repeated independent experiments. T-test: **, p < 0.01