Fig. 9
From: Alix and Syntenin-1 direct amyloid precursor protein trafficking into extracellular vesicles
Vesicular amyloid-induced reactive oxygen species are decreased following Alix and Syntenin-1 depletion. a Reactive oxygen species detected by flow cytometry following purified amyloid beta peptide or EV treatment. b Quantitation of reactive oxygen species by flow cytometry analysis over independent experiments. c Western blot analysis of EVs pre-treated with trypsin before ultracentrifugation wash and lysis. Blots are representative images from three independent experiments. MFI = mean fluorescence intensity. One-way ANOVA with post hoc Tukey’s multiple comparison test: ***, p < 0.001; **, p < 0.01