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Fig. 2 | BMC Molecular and Cell Biology

Fig. 2

From: Exonuclease resistant 18S and 25S ribosomal RNA components in yeast are possibly newly transcribed by RNA polymerase II

Fig. 2

Percentage of Terminator resistant ribosomal RNA 18S and 25S molecules, as measured by Agilent Bioanalyzer 2100. a Resistance to Terminator digestion in total RNA isolated from of C. albicans, either from mid-log (ML) and stationary growth phases and mid-log cells treated with BMH21. b Terminator resistance percentage measured in RNA isolated from nuclei under same pre-isolation conditions as in (a). c Comparison of Terminator resistance percentages in nuclear versus total RNA from cells in stationary growth phase and (d) BMH21 treated cells. For each condition three different experiments were performed. Statistical analysis was done using Agilent Bioanalyzer 2100 Expert Software. P values generated by Student’s test, *p < 0.05, **p < 0.01, ***p < 0.001. e Northern blot showing RNA extracted from C. albicans at mid-log (ML), stationary (STAT) and after BMH21 treatment. RNA was digested with Terminator (T+) or undigested (T-) and the membrane was hybridized with 25S, 18S and 5S probes (Table S1) f SYBR gold stained agarose gel that was used in Northern blot in (e)

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