Fig. 3

Presence of caps on 18S and 25S RNAs from stationary and BMH21 treated cells. a SYBR-gold stained gel showing that decapping did not affect RNA integrity. b Immunoblot of the gel (a) showing decrease in intensity after treatment with decapping enzyme. Some cross reactivity can be seen in lanes 1 and 3 (see text). c SYBR gold stained gel and corresponding Northern blot showing decapping of 18S, 25S and mRNAs. d SYBR gold stained gel showing total RNA extracted from mid-log (ML) and stationary (ST) C. albicans (lanes 2 and 3) and immunoblot (lanes 4 and 5) using anti-m7G-cap mAb to detect bands precipitated by cap binding protein (CBP) eIF4F. e Conditions in lanes 2 and 3 are the same as in (d), lanes 4 and 5 are untreated mid-log and stationary RNA. In lanes 6 and 7 RNA was decapped prior to precipitating with CBP, showing that decapping removes the target of eIF4E protein