Fig. 1From: Estrogen suppresses SOX9 and activates markers of female development in a human testis-derived cell lineERα immunofluorescence staining in NT2/D1 cells after 48 h of culture. ERα (green) was primarily cytoplasmic in control cells. EE2 treatment at both 10 nM and 100 nM led to a significant increase in nuclear ERα. Top panel shows ERα, middle panel shows DAPI, bottom panel shows a merge. Nuclei stained with DAPI; magenta. Scale bar 5 μmBack to article page