Fig. 3From: Estrogen suppresses SOX9 and activates markers of female development in a human testis-derived cell lineSOX9 immunofluorescence staining in NT2/D1 cells after 96 h of culture. SOX9 (green) was primarily nuclear (nuclei marked by DAPI; magenta) in the control cells. EE2 treatment at both 10 nM and 100 nM led to a greater amount of SOX9 in the cytoplasm. Top panel shows SOX9, middle panel shows DAPI, bottom panel shows a merge. Scale bar 5 μmBack to article page