Fig. 4

Phenotypes and genotypes of Pxkmo and Pxcardinal mutants. a and h: Wildtype phenotype. b and i: Pxkmo yellow-eye mutant phenotype. c and j: Pxkmo red-eye mutant phenotype. d-g and K-M: Pxcardinal mutant phenotypes. For Pxcardinal newly eclosed adults showed yellow compound eyes (d and k), which started to change on Day 2 or Day 3 post eclosion and gradually turned red (in the order of D to G, or K to M), although a small minority of individuals did not change. h-m: Red dashed circles indicate the positions of ocelli on increased magnification images. o: Pxkmo mutant genotypes. Two sgRNA targets are shown with red color. p: Pxcardinal mutant genotypes. Two sgRNA targets are marked with blue color. The protospacer adjacent motif (PAM) sites are underlined. Abbreviation: YE: yellow-eye; RE: red-eye. Scale bar = 0.2 mm