Fig. 1

Combination of microfluidics and microscopy. a Left: A schematic sketch of the pneumatically driven BioFlux flow chamber mounted on the top of the Zeiss Axio Observer microscope, which is operated in fluorescence mode, or reflection interference contrast mode (polarised monochromatic light). Right: Flow is driven by air pressure from the reservoir into the straight duct. Two channels can be screened simultaneously in the field of view (dashed circle). b Fluorescence microscopic image of washed platelets interacting with the footprint of two independent parallel channels under flow conditions. Both channels are coated with wt VWF. c RICM image of platelet adhesion in whole blood under flow conditions. The channel is coated with wt VWF. Scale bars correspond to 200 μm