Fig. 2

Platelet adhesion on immobilised VWF. a After biofunctionalisation of the microfluidic channels with the three different VWF A-domain deletion variants or wt VWF, the adhesion characteristics of platelets are measured upon constant shear rate application of 500 s^− 1 at indicated points in time. Representative images out of four independent experiments for each group are depicted. As expected, coating with wt VWF leads to an increasing platelet adhesion over time. Del-A1 VWF coating fails to bind platelets. The slight change of platelet adhesion to del-A2 remained negligible compared to the wt VWF. Platelet adhesion to del-A3 coating is similar to wildtype. Biofunctionalisation with BSA serves as negative control. Scale bars correspond to 100 μm. b The relative platelet surface coverage (SC) to wt VWF on indicated biofunctionalisation is plotted against time. Wt VWF (black column), del-A3 (grey column) and del-A2 (red column) show similar platelet adhesion characteristics over time. Del-A1 (green column) nearly fails to bind platelets comparable to BSA serving as negative control (data not shown). n > = 4 for each experimental group, * indicates P < 0.05