Fig. 6

LUCAT1/miR-181a-5p axis regulates the expression of KLF6 and KLF15. a and c Outputs of the online servers on the binding of miR-181a-5p with the 3’-UTR region of KLF6 and KLF15 mRNAs. b and d Validation of the binding of miR-181a-5p with KLF6 and KLF15 mRNAs by the 3’-UTR luciferase reporter gene assay. e Western blot analysis of KLF6 and KLF15 expression in MDA-MB-231 cells. f Western blot analysis of KLF6 and KLF15 expression in mice tumor tissues. g Western blot analysis of KLF6, KLF15 and ERα expression in MDA-MB-231 cells. *Compared with Ctrl group, *p < 0.05. **p < 0.01, n = 3 or 5