Fig. 3
From: Obg-like ATPase 1 inhibited oral carcinoma cell metastasis through TGFβ/SMAD2 axis in vitro
OLA1 regulates oral cancer metastasis through Smad2/Smad4 mediated EMT pathway. a mRNA fold change level of EMT markers between UM-1 siR-OLA1 cell and siR-NC cell. mRNA fold change level of EMT marker in UM-2 OLA1 knock down cells (b) and UM-1 OLA1 overexpression cells (c). d mRNA fold change level of TGFβ/SMDA2 pathway markers between UM-1 siR-OLA1 cells and siR-NC cells. mRNA fold change level of TGFβ/SMDA2 pathway markers in UM-2 OLA1 knock down cells (e) and UM-1 OLA1 overexpression cells (f). g Protein level of EMT marker in UM-1 OLA1 overexpression cells and UM-2 OLA1 knock down cells. h Protein level of TGFβ/SMDA2 pathway markers in UM-1 OLA1 overexpression cells and UM-2 OLA1 knock down cells. i Western blotting assay showed SMAD2 was silenced by siR-SMAD2 in UM-2 shOLA1 cells. j Western blotting assay showed that silenced SMAD2 decreased OLA1 expression in UM-2 cells. k Wound healing assay showed SMAD2 silenced could rescue enhanced metastasis induced by knocking down OLA1 in UM-2 cells (n = 3). l Transwell assay to examine the effect of OLA1 on metastasis by smad2 in UM-2 cells (n = 3). vs. Control, *P < 0.05, **P < 0.01, ***P < 0.001