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Fig. 8 | BMC Molecular and Cell Biology

Fig. 8

From: A fast, efficient and high-throughput procedure involving laser microdissection and RT droplet digital PCR for tissue-specific expression profiling of rice roots

Fig. 8

Expression profiling of candidate genes identified from RiceXpro in root tissues using RT-ddPCR. One-dimensional diagrams of ddRT-PCR for OsSHR1 (a), 5NG4 (b), serine palmitoyltransferase (c), disease resistance response protein (d) and pollen Oe1 (e) in three tissues (stele, cortex, and epidermis/exodermis) and a negative control (H2O). The expression profiles extracted from the RiceXpro database are shown in parallel. OsSHR1 was used as a positive control of stele-specific expression. EXP’ was used as a constitutive control for ddRT-PCR, excepted for OsSHR1 and serine palmitoyltransferase as the probes for these two genes had the same FAM fluorophore as EXP’

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BMC Molecular and Cell Biology

ISSN: 2661-8850

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