Fig. 9From: A fast, efficient and high-throughput procedure involving laser microdissection and RT droplet digital PCR for tissue-specific expression profiling of rice rootsRelative expression profiling of 5NG4 in the stele, cortex and epidermis/exodermis using TFIIE and EXP’ for normalization of RNA quantity. 1D diagram of ddRT-PCR for 5NG4 expression in three tissues (stele, cortex and epidermis/exodermis) and a negative control (water) using EXP’ (a) and TFIIe (b) for normalization. Blue, FAM probes; green, HEX probes. Bottom, relative expression levels in the three tissues after normalization with EXP’ (left) or TFIIE (right). The abscises represent arbitrary expression valuesBack to article page